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ART: ASSISTED REPRODUCTIVE TECHNOLOGY

COLLECTION AND TREATMENT OF SPERM FOR ARTIFICIAL INSEMINATION. The Biological Centre is authorized to collect and treat the sperm in preparation for artificial insemination with the donor’s partner.

  • PMA : Procréation médicalement assistée
  • Trisomie 21
Main reasons for intra-cervical insemination with partner’s sperm

-Poor quality cervical mucus
-Oligospermia – moderate sperm count
-Unexplained infertility
-Ejaculation problems

Preliminary meeting before using partner’s sperm for insemination

It is essential that all requests for artificial insemination using a partner’s sperm pass via a preliminary meeting with a qualified biologist from the laboratory (law 11 April 2008 & 03 August 2010 concerning the rules for correct clinical and biological practices for artificial insemination)

This meeting will take place at the laboratory on 16 rue des 4 coins, it will be organized after the first tests (blood checks and sperm analysis) and at least one month before the date for insemination. The aim of the interview is to learn the medical history for the couple and to explain the technique used for preparing the sperm. A medical questionnaire will be used by the biologist during the meeting in order to check the reasoning behind the artificial insemination request.

The couple should ensure that they have with them:

  • Photocopy of their marriage certificate (livret de famille) or proof that they have lived together for at least 2 years.
  • Photocopies of the identity cards or passports
  • Identity photos
  • Proof of address
  • A written request from the gynecologist for assisted fertility treatment.
  • Written consent from the gynecologist for assisted fertility treatment.
  • Results from all tests that have been carried out so far in relation to the request in particular serological testing.

The meeting will take place with one of the biologists authorized by the medical laboratory: Dr Philippe Andlauer or Dr Eric Gaeremynck

The patient will then follow a treatment prescribed by the gynecologist to stimulate the development of follicles in the ovaries. Each follicle contains an oocyte or ovule.

The treatment is usually carried out via subcutaneous injections which can be carried out by a nurse or by the patient herself. In both cases it is extremely important that the hour and measures be followed to the letter. The patient must inform the gynecologist of any error or oversight.

Various methods of control are used to check the level of ovarian stimulation via ultrasound and blood tests (LH hormone levels, ESTRADIOL, PROGESTERONE), during the last control the gynecologist will prescribe a last injection (HCG or OVITRELLE) that will cause the patient to ovulate 37hours later.

Again it is essential to respect the hour of the last does so that the insemination can take place right up to the moment when the patient will ovulate.

After insemination

14 days after insemination a pregnancy test is carried out.

The laboratory must be informed of the test result (positive or negative) because laboratories who prepare sperm for insemination must provide a yearly report to the BIOMEDICINE agency who will evaluate their performance.

Collection and Treatment of Sperm
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Once the date for insemination has been determined by the gynecologist you must immediately make an appointment at the laboratory.

Collecting the sperm

- An identity check will be carried out just before collection of the sperm
- The collection is carried out in the laboratory in a dedicated room with or without the partner
- Respect a delay of abstinence (abstain from ejaculation) 2 to 7 days
- Urinate just before the collection so as to reduce the risk of bacterial contamination from the urethra
- Carry out careful cleaning of the shaft and the hands : water-soap-antiseptic
- The sperm must be collected via masturbation into a sterile container.

Treating the sperm in the laboratory

This consists of selecting the most mobile spermatozoids and the most apt – which means spermatozoids that have the best chemico-physical characteristics for fertilizing the ovules (oocytes). This technique also enables the elimination of any debris cells or toxic factors in the seminal plasma.